Figure and Table | Advancements in Whole-Genome Sequencing Protocols: A Decade of In-House Operations and Quality Controls at the Tohoku Medical Megabank

Table 1. The list of WGS Projects Conducted around 2015.

Genome projects	Sample number	Average coverage	Sample number with over 30×	Publication date
ToMMo, Japan (this project)	1,070	32.4×	1,070	August 2015^a
deCODE, Iceland	2,636	20×	909	May 2015^b
1000 Genome project phase 3	2,504	7.4×	453	September 2015^c
UK10K project	3,781	7×	0	September 2015^d
SardiNIA project, Italy	2,120	4×	0	September 2015^e
BBMRI, The Netherlands	750	12×	0	February 2014^f
African Genome Variation	320	4×	0	December 2014^g

Table 2. Sequencing Platforms and Protocols Used for the TMM 100,000 WGS Project.

Platforms	Mode	Protocol	Output	Approximate number
Platforms	Mode	Protocol	Output	of samples analyzed
HiSeq 2500	Rapid Run	162PE	97Gb × 2	3,400
HiSeq 2500	Rapid Run	259PE	155Gb × 2	300
HiSeq X	N/A	150PE	900Gb × 2	<100
DNBSEQ-G400	FCL	150PE	540Gb × 2	1,400
DNBSEQ-T7	N/A	150PE	1.5Tb × 4	8,400
NovaSeq 6000	S4 flowcell	150PE	3Tb × 2	24,800
		151PE	3Tb × 2	4,900
		161PE	3.2Tb × 2	48,000
NovaSeq X Plus	10B flowcell	161PE	3Tb × 2	9,000
NovaSeq X Plus	25B flowcell	161PE	8Tb × 2	9,000