JMA Journal

Figure 1. Relative abundance of target-vertebrate animal in each vector.
Each bar graph represents a sequence derived from an individual T. dimidiata. The bar graph is color-coded by species and is expressed as a percentage of the number of reads of each triatomine.
T. dimidiata: Triatoma dimidiata.

Figure 2. Proportions of blood source species in all triatomines and four categorized groups based on capture location and life stage.
The proportions of blood source species were calculated based on the absolute number of sequences. A significant difference was observed in the components of blood source species between domicile and peridomestic groups (Wilcoxon rank-sum test, p = 0.006).
* Less likely host

Figure 3. Human blood ingestion rate for different geographical locations.
The radius of the pie chart for each department reflects the number of triatomines.

Figure 4. Relative abundance of four representative vertebrates in each sample.
The number of sequences derived from individual vectors is used as the denominator, and each bar graph shows the sequence percentages for each animal in decreasing order.

Figure 5. Implications of less likely hosts on T. cruzi infection rate.
(a) Statistical considerations in the relative abundance of the less likely host (n = 131)
We compared the relative abundance of less likely hosts with three vertebrates in two groups. The black triangle group has a T. cruzi infection rate per household of less than 33% (n = 79); the other has a T. cruzi infection rate per household of 33% and over (n = 52). One plot represents an individual vector. A significant difference was observed in less likely host abundance between the two groups (Mann-Whitney test, p = 0.0007).
(b) Household variety of T. cruzi infection rate and relative abundance of less likely hosts (n = 131).
We determined the correlation matrix for T. cruzi infection rate and less likely host abundance (n = 131). One plot represents an individual vector. A weak negative correlation was observed (Spearman’s correlation coefficient, r_s = −0.28, p = 0.001) between the infection rate of collected vectors calculated in each household and less likely host abundance.
T. cruzi: Trypanosoma cruzi.

Figure 6. Feeding and possible parasite transmission network.
Feeding networks were described to visualize possible transmission pathways of T. cruzi and the frequency of the respective blood-feeding sources. Animals indicated by black circles are known hosts and animals indicated by gray triangles are less likely hosts. The thickness of the line connecting the vertebrates reflected the detection frequency of the vertebrates.
T. cruzi: Trypanosoma cruzi.