Figure 1. A: HEK293 cells expressing the RBM20 variants (R634W and S635A) were generated separately. The green arrowhead indicates the nicking site, whereas the NGG (blue box) denotes the PAM sequence for SpCas9 used in PE.
B: PE corrected multiple mutations within the RSRSP region, including R634W and S635A. The R634W mutation (TGG) was corrected to CGC, encoding arginine (R), whereas the S635A mutation (GCT) was corrected to TCT, encoding serine (S).
PE: prime editing.
From: Prime Editing Corrects Multiple Mutations in the RSRSP Region of the RBM20 Gene Using a Single Prime Editing Guide RNA
