Table 1. Detection of Viral DNA in Nails by Real-Time PCR.

From: Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection

Virus Number of patients (%)
Hepatitis B virus 37 (62)
Herpes simplex virus 1 0
Herpes simplex virus 2 0
Varicella-zoster virus 0
Epstein‒Barr virus 0
Cytomegalovirus 0
Human herpes virus 6 0
Human herpes virus 7 3 (5)
Table 2. Nail Samples for Capture-Based Next-Generation Sequencing.

From: Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection

ID Gender Age HBeAg HBV genotype Level of HBV DNA in serum (Log copies/mL) Level of HBV DNA in nail (Log copies/mL) Level of HHV-7 DNA in nail (Log copies/mL) Concentration of extracted tissue DNA (ng/μL)*
Ig18203 M 5 Positive C 7.8 3.3 2.7 21
Ig18204 M 10 Positive C >9.0 2.3 3.0 10.3
Ig18205 M 8 Positive B 8.9 3.8 2.8 8.2
Table 3. Integration Breakpoints of the Positive Controls.

From: Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection

ID Total number of integration breakpoints
HBV HHV-7
Ig18206
(liver)
618 0
A breakpoint was detected in 1 read: 488 points
The same breakpoint was detected in
2 reads: 84 points
3 reads: 39 points
4 reads: 20 points
5 reads: 12 points
6 reads: 4 points
7 reads: 5 points
8 reads: 1 point
9 reads: 2 points
10 reads: 1 point
13 reads: 1 point
14 reads: 1 point
Ig18807
(liver)
15,620 9
A breakpoint was detected in 1 read: 14,950 points A breakpoint was detected in 1 read: 9 points
The same breakpoint was detected in
2 reads: 288 points
3 reads: 34 points
4 reads: 10 points
5 reads: 7 points
6 reads: 7 points
7 reads: 3 points
8 reads: 5 points
10 reads: 4 points
11 reads: 1 point
2 reads: 1 point
4 reads: 1 point
5 reads: 2 points
22 reads: 1 point
24 reads: 1 point
30 reads: 1 point
42 reads: 1 point
51 reads: 1 point
53 reads: 1 point
73 reads: 1 point
Ig18207
(whole blood)
48 6
A breakpoint was detected in 1 read: 42 points A breakpoint was detected in 1 read: 6 points
The same breakpoint was detected in 2 reads: 6 points
Table 4. Integration Breakpoints of the Negative Controls.

From: Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection

ID Total number of integration breakpoints
HBV HHV-7
Ig18208
(nails)
1 0
A breakpoint was detected in 1 read: 1 point
Ig18808
(liver)
0 1
A breakpoint was detected in 1 read: 1 point
Table 5. Integration Breakpoints of the Three Nail Samples.

From: Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection

No. Total number of integration breakpoint
HBV HHV-7
Ig18203 7 7
A breakpoint was detected in 1 read: 5 points A breakpoint was detected in 1 read: 6 points
The same breakpoint was detected in 2 reads: 1 point The same breakpoint was detected in 2 reads: 1 point
The same breakpoint was detected in 3 reads: 1 point
Ig18204 0 37
A breakpoint was detected in 1 read: 29 points
The same breakpoint was detected in 2 reads: 7 points
The same single breakpoint was detected in 3 reads: 1 point
Ig18205 0 59
A breakpoint was detected in 1 read: 50 points
The same breakpoint was detected in 2 reads: 7 points
The same breakpoint was detected in 4 reads: 2 points
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